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The course provides training in the methods of light and fluorescence microscopy, good laboratory practice and
rules governing the organization of a biological experiment. The course objectives further include: inspection of
structure of plant and animal cells and tissues, microscopy and cytochemistry of cellular processes in practical
and theoretical tasks. This course not only develops students' spatial imagination, but also teaches them to abstract
and record important scientific information of the reality observed in the microscope.
Last update: MATOUSKJ (23.02.2022)
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Students will be able to:
Describe principles the light and fluorescence microscopy lie in and will be knowledgeable in principal parameters of a light microscope.
Independently prepare microscope specimens, inspect them through the light and fluorescence microscopy, organize an experiment and properly record and interpret the observations.
Better understand organization of the cell, plant and animal tissues, mitosis and chromosomal basis of heredity, which were all in theory the subjects of preceding Biology course. Last update: Kotrba Pavel (17.01.2018)
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Passing the exam consisting of a written and oral examination. Formally and substantively correct protocol on samples observed in the practical course. Last update: MATOUSKJ (04.02.2022)
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Z:Kotrba P., Babůrek I., Knejzlík Z., Návody ke cvičením z biologie, VŠCHT Praha, 2006, 8070806230
D:Benda V., Babůrek I., Kotrba P., Základy biologie, VŠCHT Praha, 2005, 8070805870 Last update: Kotrba Pavel (17.01.2018)
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Attendance at laboratory courses Last update: Kotrba Pavel (17.01.2018)
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1 a) Introduction to light microscopy, fluorescence microscopy, and other microscopic techniques 1 b) Preparation of samples for light microscopy 1 c) Microscopy of plant epidermal structures and pollen grains
2 a) Cytology and imaging methods of subcellular structures 2 b) Microscopy of plant organelles and calcium oxalate crystals 2 c) Protoplasmic streaming and osmotic phenomena in the cell
3 a) Plant anatomy and organology 3 b) Plant tissues and their imaging 3 c) Microscopic structure of the stem, root, and leaf
4 a) Cytochemistry and the cell cycle 4 b) Microscopy of the stages of mitosis in the meristem 4 c) Visualization of plant polymers and enzyme functionality
5 a) Basics of human cell histology 5 b) Microscopic comparison of healthy and pathologically altered tissues Last update: MATOUSKJ (26.01.2023)
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Anonymous, Boundless Biology Open Textbook, boundless.com, free acess at https://www.oercommons.org/authoring/54851-boundless-biology-textbook/1/view Last update: MATOUSKJ (10.02.2023)
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none Last update: Kotrba Pavel (17.01.2018)
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Teaching methods | ||||
Activity | Credits | Hours | ||
Účast v laboratořích (na exkurzi nebo praxi) | 1 | 28 | ||
1 / 1 | 28 / 28 |