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The course provides training in the methods of light and fluorescence microscopy, good laboratory practice and
rules governing organization of a biological experiment. The course objectives further include: inspection of
structure of plant and animal cells and tissues, microscopy and cytochemistry of cellular processes, and analysis of
chromosomal basis of heredity.
Last update: Kubová Petra (06.05.2019)
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Students will be able to:
Describe principles the light and fluorescence microscopy lie in and will be knowledgeable in principal parameters of a light microscope.
Independently prepare microscope specimens, inspect them through the light and fluorescence microscopy, organize an experiment and properly record and interpret the observations.
Better understand organization of the cell, plant and animal tissues, mitosis and chromosomal basis of heredity, which were all in theory the subjects of preceding Biology course. Last update: Kubová Petra (06.05.2019)
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Passing the exam consisting of a written and oral examination. Last update: Kubová Petra (06.05.2019)
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Z:Kotrba P., Babůrek I., Knejzlík Z., Návody ke cvičením z biologie, VŠCHT Praha, 2006, 8070806230
D:Benda V., Babůrek I., Kotrba P., Základy biologie, VŠCHT Praha, 2005, 8070805870 Last update: Kubová Petra (06.05.2019)
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Attendance at laboratory courses Last update: Kubová Petra (06.05.2019)
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1. Introduction to Light Microscopy, Fluorescence Microscopy and Other Microscopy Techniques
2. Specimen Preparation for Light Microscopy
3. Microscopy of the Cell, Morphology and Inclusion Bodies of the Cell
4. Microscopy of Organelles of the Cell with Light Microscope
5. Microscopy of Organelles of the Cell using Fluorescence Microscopy
6. Cytochemical Staining of Macromolecules and Detection of Enzyme Activity
7. Osmosis in Cells
8. Cell Types and Intercellular Space in Plant Tissues
9. Anatomy of Stems, Roots and Leaves and Vascular Tissue in Plants
10. Histology � Epithelial and Connective Tissues, Blood Smear
11. Histology � Muscle and Nervous Tissues
12. Cytogenetics � Animal Chromosomes and Karyotype
13. Mitosis in Plant Meristem and Cells of Animal Tissue Culture
14. Exercises in Plant, Animal and Human Genetics Last update: Kubová Petra (06.05.2019)
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Anonymous, Boundless Biology Open Textbook, boundless.com, free acess at https://www.boundless.com/biology/ Last update: Kubová Petra (06.05.2019)
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none Last update: Kubová Petra (06.05.2019)
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