Studenti budou umět:

Základní pojmy molekulové genetiky, vztah struktury a funkce nukleových kyselin.

Principy regulace předávání genetické informace a její proměnlivosti v důsledku mutací a rekombinací.

Mechanismy pro opravu mutací, kterými organismy disponují.

Základní kontrolní procesy genové exprese od regulace transkripce u prokaryot a eukaryot a posttranskripční modifikace eukaryotické mRNA, až po regulaci stability mRNA a translaci, role malých RNA.

Základní principy práce s DNA, metody analýzy DNA a základní aplikace polymerasové řetězové reakce

Students will know:

Basic principles of molecular genetics and the relationship of their structure and function.

Principles of regulation of transfer of genetic information and its variability due to mutations and recombination

Mechanisms involved in mutation repair.

Basic processes of gene expression as regulation of transcription, in prokaryotes and eukaryotes and posttranscriptional modifications of eukaryotic mRNA, regulation of stability of mRNA and translation, role of small RNAs.

Basic principles of work with DNA, methods of DNA analysis and basic applications of polymerase chain reaction

Michael J Simmons, Peter D. Snustad: Genetika, Nakladatel: Masarykova univerzita Brno 2017

Lodish, H.; et al. Molecular cell biology, 8th ed.;W.H.Freeman & Co Ltd, 2016.

Albrets B; et al. Molecular biology of the cell, 5th ed. Garland Science, 2008.

Clark D.P. Molecular biology, Elsevier Inc., 2005.

Hitler J.M. Forensic DNA Typing, 2nd ed. Elsevier 2005.

Michael J Simmons, Peter D. Snustad: Genetika, Nakladatel: Masarykova univerzita Brno 2017

Lodish, H.; et al. Molecular cell biology, 8th ed.;W.H.Freeman & Co Ltd, 2016.

Albrets B; et al. Molecular biology of the cell, 5th ed. Garland Science, 2008.

Clark D.P. Molecular biology, Elsevier Inc., 2005.

Hitler J.M. Forensic DNA Typing, 2nd ed. Elsevier 2005.

žádné

none

1. Historical overview, basic genetic concepts and laws

2. Structure and function of nucleic acids, genome organization

3. Replication I, general features, prokaryotes

4. Replication II, eukaryotes, replisome complex

5. Recombination I, gene segregation in meiosis

6. Recombination II, transposition

7. Mutation and repair mechanisms

8. Methods of DNA sequence analysis, genome mapping

9. Regulation of transcription

10. Posttranscriptional modification of RNA

11. Translation I, initiation, elongation and termination, differences between prokaryotes and eukaryotes

12. Translation II, posttranslational modifications

13. Basic principles of pharmacogenomics

14. Basics of oncogenesis

Pro zápis tohoto předmětu je nutno mít minimálně zapsány předměty N110004 A N320004

To enroll in the course it is required enrollment in courses N110004 A N320004

Biochemie I

Biochemistry I