Students will be able to:

Understand the biological, chemical and engineering processes in the biotechnology industry

Describe and evaluate basic biocatalytic processes.

Quantitatively evaluate experimental data of processes catalyzed by biological elements and to carry out heat and mass balances of biotechnological processes.

Design mixing and aeration equipment of bioreactors.

Students will be able to:

Understand the biological, chemical and engineering processes in the biotechnology industry

Describe and evaluate basic biocatalytic processes.

Quantitatively evaluate experimental data of processes catalyzed by biological elements and to carry out heat and mass balances of biotechnological processes.

Design mixing and aeration equipment of bioreactors.

R: Doran P.M.: Bioprocess Engineering Principles, Academic, London, 1995, ISBN: 9780122208553

R: Moo-Young M.: Comprehensive biotechnology, Elsevier B.V., 2011, ISBN: 9780080885049

A: Dunn I.J. et al.: Biological Reaction Engineering, Wiley-VCH, 2003, ISBN:9783527307593

A: Najafpour G.D.: Biochemical Engineering and Biotechnology, Elsevier B.V., 2007, ISBN 9780444528452

R: Doran P.M.: Bioprocess Engineering Principles, Academic, London, 1995, ISBN: 9780122208553

R: Moo-Young M.: Comprehensive biotechnology, Elsevier B.V., 2011, ISBN: 9780080885049

A: Dunn I.J. et al.: Biological Reaction Engineering, Wiley-VCH, 2003, ISBN:9783527307593

A: Najafpour G.D.: Biochemical Engineering and Biotechnology, Elsevier B.V., 2007, ISBN 9780444528452

1. Scope of biotechnology

2. Scope of bioengineering

3. Enzyme kinetics

4. Kinetics of cell growth and product formation

5. Factors affecting the growth rate

6. Bioprocess characteristics

7. Cell cultivation techniques

8. Continuous cultivations

9. Control systems - Chemostat and Turbidistat

10. One-stage and multi-stage continuous culture systems

11. Mixing in Biological Reactors

12. Aeration and oxygen transfer in bioreactors

13. Bioreactors

14. Practical applications of bioreactors

1. The issue of biotechnology and bioengineering.

2. The structure of bioprocess, equipment overview and unit operations.

3. Methods of optimization and modeling of biological processes.

4. Engineering kinetics of chemical and enzymatic reactions, kinetics of microbial growth.

5. Cultivation of microorganisms - batch and fed-batch cell cultures.

6. Cultivation of microorganisms - semicontinuous and continuous cell cultures.

7. Characteristics of phase folw - residence time distribution, flow models.

8. Mass transfer - oxygen transfer, aeration, measurement and quantification of KLa.

9. Specific aspects of plant/tissue cell and phototrophic cell cultivation.

10. Engineering aspects of enzyme and cell immobilization, biocatalysis.

11. Mixing in bioreactors - theory, power consumption, homogenization, shear forces.

12. Heat transfer, energy balance of bioreactors, sterilization, cooling and heating.

13. Scale-up of bioprocesses.

14. Construction materials, corrosion, design and construction of biotechnological processes.

http://eso.vscht.cz/predmety/N319008/

http://eso.vscht.cz/predmety/N319008/