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1. Introduction, cell disruption, filtration, ultrafiltration, dialysis 2. Precipitation, extraction, centrifugation (preparative, analytical), isolation of nucleic acid 3. Introduction to the chromatography, instrumentation and theory of chromatography 4. Gel chromatography 5. Ion exchange chromatography, chromatofocusation 6. Hydrophobic chromatography and reverse phase 7. Affinity chromatography 8. Recombinant proteins and their purification 9. Thin layer chromatography and recovery and field of chromatography 10. Introduction to the electrophoretic methods, SDS, native electrophoresis, blotting 11. 2D electrophoresis, isoelectric focusation 12. Pulse electrophoresis, capillary electrophoresis 13. Immobilisation of enzymes Immobilisation of cell Poslední úprava: KNOBLOCL (11.04.2012)
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